What is the half-life of Humanin?

Humanin has a half-life of ~30 minutes (native); enhanced analogs (HNG) have longer duration, which is why it's typically administered Once daily or 3-5 times per week for optimal therapeutic effect.

How do I reconstitute Humanin?

Add bacteriostatic water to the peptide vial slowly down the side. Typical reconstitution uses 2-3ml of water for a 5mg vial. Gently swirl, do not shake.

Anti-Ageing

Preclinical

Humanin

Also known as: HN, HNG, S14G-Humanin, Mitochondrial-Derived Peptide

Overview

Key Facts

Primary Goal: A 24-amino-acid mitochondrial-derived peptide encoded in the 16S ribosomal RNA gene of mitochondrial DNA

A 24-amino-acid mitochondrial-derived peptide encoded in the 16S ribosomal RNA gene of mitochondrial DNA. Humanin is a potent cytoprotective factor that protects cells against apoptosis and stress, with particular relevance to neurodegenerative diseases, cardiovascular protection, and metabolic health. Its levels decline with age, correlating with age-related disease onset. Half-Life ~30 minutes (native); enhanced analogs (HNG) have longer duration Typical Dose 1–5 mg Frequency Once daily or 3-5 times per week Cycle Length 4-8 weeks on, 4 weeks off

Dosing Information

Half-Life

~30 minutes (native); enhanced analogs (HNG) have longer duration

Typical Dose

1–5 mg

Frequency

Once daily or 3-5 times per week

Cycle Length

4-8 weeks on, 4 weeks off

Administration Routes:

subcutaneousintravenous

Storage & Reconstitution

Unreconstituted (Powder)

Temperature2–8°C (36–46°F) or -20°C (-4°F) for long-term

DurationUp to 3 months

Reconstituted (Mixed)

Temperature2–8°C (36–46°F)

Duration2-4 weeks

Research Summary

Preclinical

Discovered in 2001 by Bhshimoto et al. during research on Alzheimer's disease, humanin has since been shown to have broad cytoprotective effects. The S14G analog (HNG) is 1000x more potent than native humanin. Studies show that circulating humanin levels decline with age and correlate with Alzheimer's risk, mitochondrial dysfunction, and metabolic disease. Animal studies demonstrate protection against atherosclerosis, diabetes, and neurodegeneration. Epidemiological data from centenarian studies (led by Dr. Pinchas Cohen at USC) show elevated humanin levels in exceptionally long-lived individuals.