What is the half-life of NAD+?

NAD+ has a half-life of ~30-45 minutes (IV); tissue levels persist longer, which is why it's typically administered 1-3x per week (subcutaneous) or 250-500 mg IV for optimal therapeutic effect.

How do I reconstitute NAD+?

Add bacteriostatic water to the peptide vial slowly down the side. Typical reconstitution uses 2-3ml of water for a 5mg vial. Gently swirl, do not shake.

Anti-Ageing

Preclinical

NAD+

Also known as: NAD, Coenzyme NAD, Nicotinamide Adenine Dinucleotide

Overview

Key Facts

Primary Goal: A critical coenzyme found in every cell of the body, essential for energy metabolism, DNA repair, sirtuin activation, and cellular signaling

A critical coenzyme found in every cell of the body, essential for energy metabolism, DNA repair, sirtuin activation, and cellular signaling. NAD+ levels decline approximately 50% between ages 40 and 60, contributing to metabolic dysfunction, neurodegeneration, and accelerated aging. Exogenous NAD+ supplementation — via IV infusion, subcutaneous injection, or precursors (NMN, NR) — has become one of the most popular anti-aging interventions. Half-Life ~30-45 minutes (IV); tissue levels persist longer Typical Dose 50–200 mg Frequency 1-3x per week (subcutaneous) or 250-500 mg IV Cycle Length Continuous supplementation or 4-12 week cycles

Dosing Information

Half-Life

~30-45 minutes (IV); tissue levels persist longer

Typical Dose

50–200 mg

Frequency

1-3x per week (subcutaneous) or 250-500 mg IV

Cycle Length

Continuous supplementation or 4-12 week cycles

Administration Routes:

subcutaneousintramuscularoralintravenous

Storage & Reconstitution

Unreconstituted (Powder)

Temperature2–8°C (36–46°F)

DurationUp to 3 months

Reconstituted (Mixed)

Temperature2–8°C (36–46°F)

Duration2-4 weeks

Research Summary

Preclinical

NAD+ biology has exploded in research interest since the work of David Sinclair (Harvard) and Shin-ichiro Imai (Washington University) on sirtuins and aging. Human studies have demonstrated that NAD+ precursors (NMN and NR) safely elevate blood NAD+ levels. Clinical trials show improvements in insulin sensitivity, muscle function in elderly subjects, and cardiovascular biomarkers. IV NAD+ is widely used in integrative medicine clinics for energy, cognitive support, and addiction recovery, though large-scale RCTs for the injectable form are still limited. The compound is central to the 'Information Theory of Aging' proposed by Sinclair's lab.